Correlation-Based Nanometric Localization Using Lattice SIM² and dSTORM in PML Nuclear Bodies validated by Calibration Spheres
Résumé
Optimized Lattice SIM 2 parameters to reveal the ring-like circular morphology of Promyelocytic Leukemia Nuclear Bodies (PML NBs) Correlation of Lattice SIM 2 to Lattice SIM and to dSTORM imaging using biological samples and fluorescent spheres. Investigated the effectiveness of Lattice SIM 2 to minimize Z-elongation compared to Lattice SIM Correlative Lattice 3D SIM 2 -STORM nanoscopy with Eternity-SIM buffer to minimize refractive index differences.
Authorship: All authors should have made substantial contributions to all of the following:
(1) the conception and design of the study (KM, PL, FJ, AF, CP), Sample preparation (CR, RN, PC), acquisition of data (CR, RN, KM, EC), or analysis (CR, RN, KM), interpretation of data (CR, RN, KM, PL, FJ, CP) ;(2) drafting the article (CP, KM, FJ) or revising it critically for important intellectual content (CP, KM, PL, AF) ; (3) final approval of the version to be submitted (all).
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Sciences du Vivant [q-bio]Origine | Fichiers produits par l'(les) auteur(s) |
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