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Imagerie multidimensionnelle en mode de résonance de plasmons de surface de structures de biopuces : expérience et modélisation

Mohamed Nakkach 1 
1 Laboratoire Charles Fabry / Biophotonique
LCF - Laboratoire Charles Fabry
Abstract : During this work we added the spectral parameter to the homemade Surface Plasmon Resonance Imaging (SPRI) instrument, developing an angulo-spectral instrumental system. To validate our set-up, we first used a dielectric medium absorbing in the visible spectral range as a model case. The complex dielectric function was treated by the Lorentz model and the relationship between the imaginary and the real part of the refractive index medium was calculated with the Kramers-Kronig relation. We started by injecting an absorbing dye at 630 nm in the microfluidic cell and then measured the angulo-spectral reflectivity. The experimental measurements were fitted with calculations issued from a newly developedprogram to extract the dielectric optical parameter from the experimental data. This fit calculates the imaginary and real part of the absorbing dielectric medium demonstrating the possibility for spectroscopic applications. We also integrated DNA molecules tagged with different chromophores to study the effect of the absorption wavelength on the surface plasmon angulo-spectral reflectivity variation. Furthermore, to illustrate the interest of the spectral parameter, one can integrate adielectric or metallic grating on the prism surface. Specifically, we fabricated a dielectric grating of PMMA with 250 nm period and 300 nm thickness. We observed plasmons bandgap located at 735 nm. This experimental study was in good agreement with a simulation obtained using a RCWA method based program.
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Submitted on : Monday, September 24, 2012 - 11:32:20 AM
Last modification on : Saturday, June 25, 2022 - 11:12:48 AM
Long-term archiving on: : Tuesday, December 25, 2012 - 4:15:13 AM


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  • HAL Id : tel-00734650, version 1



Mohamed Nakkach. Imagerie multidimensionnelle en mode de résonance de plasmons de surface de structures de biopuces : expérience et modélisation. Autre [cond-mat.other]. Université Paris Sud - Paris XI, 2012. Français. ⟨NNT : 2012PA112145⟩. ⟨tel-00734650⟩



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